TY - JOUR
T1 - Small-angle X-ray scattering
T2 - A bridge between RNA secondary structures and three-dimensional topological structures
AU - Fang, Xianyang
AU - Stagno, Jason R.
AU - Bhandari, Yuba R.
AU - Zuo, Xiaobing
AU - Wang, Yun Xing
N1 - Funding Information:
We sincerely thank David Lilley, Samuel Butcher, Marc Jamin, Norman Pace, Alexei Kazanstev, Ajaykumar Gopal and William Gelbart for providing materials for figures. This work was supported by the Intramural Research Program of the National Institutes of Health , National Cancer Institute (NCI) Center for Cancer Research . Use of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under contract no. DE-AC02-06CH11357 and by NCI under the PUP-77 Agreement between NCI and Argonne National Laboratory .
Publisher Copyright:
© 2015.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Whereas the structures of small to medium-sized well folded RNA molecules often can be determined by either X-ray crystallography or NMR spectroscopy, obtaining structural information for large RNAs using experimental, computational, or combined approaches remains a major interest and challenge. RNA is very sensitive to small-angle X-ray scattering (SAXS) due to high electron density along phosphate-sugar backbones, whose scattering contribution dominates SAXS intensity. For this reason, SAXS is particularly useful in obtaining global RNA structural information that outlines backbone topologies and, therefore, molecular envelopes. Such information is extremely valuable in bridging the gap between the secondary structures and three-dimensional topological structures of RNA molecules, particularly those that have proven difficult to study using other structure-determination methods. Here we review published results of RNA topological structures derived from SAXS data or in combination with other experimental data, as well as details on RNA sample preparation for SAXS experiments.
AB - Whereas the structures of small to medium-sized well folded RNA molecules often can be determined by either X-ray crystallography or NMR spectroscopy, obtaining structural information for large RNAs using experimental, computational, or combined approaches remains a major interest and challenge. RNA is very sensitive to small-angle X-ray scattering (SAXS) due to high electron density along phosphate-sugar backbones, whose scattering contribution dominates SAXS intensity. For this reason, SAXS is particularly useful in obtaining global RNA structural information that outlines backbone topologies and, therefore, molecular envelopes. Such information is extremely valuable in bridging the gap between the secondary structures and three-dimensional topological structures of RNA molecules, particularly those that have proven difficult to study using other structure-determination methods. Here we review published results of RNA topological structures derived from SAXS data or in combination with other experimental data, as well as details on RNA sample preparation for SAXS experiments.
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U2 - 10.1016/j.sbi.2015.02.010
DO - 10.1016/j.sbi.2015.02.010
M3 - Review article
C2 - 25765781
AN - SCOPUS:84924290425
SN - 0959-440X
VL - 30
SP - 147
EP - 160
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -