Slowing of Cell Cycle Traverse for Cells in Exponential Monolayer Cultures Placed into Plateau-fed and Starved Medium

David C. Allison, Kallol K. Bose, Susan Anderson, Steven Curley, Joel Robertson

Research output: Contribution to journalArticlepeer-review

Abstract

MCa-11 tumor cells in exponential monolayer cultures were pulse/ chase-labeled with [3H)thymidine and then regrown in fresh, plateau-fed, or starved medium. We measured the DNA content and autoradiographic labeling of these cells by absorption cytophotometry at intervals of 0, 2, 4,8,12, and 24 h to follow the progress through the cell cycle of those cells which had incorporated isotope. We found that for the cells grown in plateau-fed and starved medium the G0/Gi, S, and G2 phases of the cell cycle were prolonged when compared to those for cells grown in fresh medium. These results show that, under adverse microenvironmental conditions, the growth of tumor cells can be regulated in all phases of the cell cycle, and that this regulation can include lengthening and even cessation of replicative DNA synthesis.

Original languageEnglish (US)
Pages (from-to)1456-1464
Number of pages9
JournalCancer Research
Volume49
Issue number6
StatePublished - Mar 15 1989

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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