TY - JOUR
T1 - Slow virus infection
T2 - Replication and mechanisms of persistence of visna virus in sheep
AU - Narayan, O.
AU - Griffin, D. E.
AU - Silverstein, A. M.
N1 - Funding Information:
This work was supported by grants no. 5 ROI NS 12127-02, 2 POI NS 10920-04, and H.D. 07935 from the U.S. Public Health Service, and by a gift from the Hamilton Roddis Foundation. Dr. Griffin is an investigator for the Howard Hughes Medical Institute, and Dr. Silverstein is a research professor for the Independent Order of Odd Fellows.
PY - 1977
Y1 - 1977
N2 - The influence of the age and immune status of the host on the slow replication and persistence of visna virus in sheep was studied. A total of 25 randomly bred fetal lambs were inoculated intracerebrally with visna virus; 8 of the fetuses being immunosuppressed by thymectomy and lymphocyte antiserum before inoculation. The fetuses were sacrificed sequentially, and tissues were processed for viral quantitation. No exponential increase of virus occurred in either the normal or immunosuppressed fetuses, and virus was recovered mainly by explantation of tissues. This indicated that the viral genome was present in tissue cells but that the extent of replication in the early phase of infection was restricted by factors unassociated with the maturation or immune status of the host. In addition, virus isolated from the peripheral blood leukocytes of a sheep 1 yr after inoculation was antigenically distinct from the plaque purified virus used for inoculation. Furthermore, the sheep serum neutralized the input virus but not the new isolate. Rhis distinction suggested that a major antigenic shift of the agent had occurred and had provided another mechanism for the maintenance of the persistent infection.
AB - The influence of the age and immune status of the host on the slow replication and persistence of visna virus in sheep was studied. A total of 25 randomly bred fetal lambs were inoculated intracerebrally with visna virus; 8 of the fetuses being immunosuppressed by thymectomy and lymphocyte antiserum before inoculation. The fetuses were sacrificed sequentially, and tissues were processed for viral quantitation. No exponential increase of virus occurred in either the normal or immunosuppressed fetuses, and virus was recovered mainly by explantation of tissues. This indicated that the viral genome was present in tissue cells but that the extent of replication in the early phase of infection was restricted by factors unassociated with the maturation or immune status of the host. In addition, virus isolated from the peripheral blood leukocytes of a sheep 1 yr after inoculation was antigenically distinct from the plaque purified virus used for inoculation. Furthermore, the sheep serum neutralized the input virus but not the new isolate. Rhis distinction suggested that a major antigenic shift of the agent had occurred and had provided another mechanism for the maintenance of the persistent infection.
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U2 - 10.1093/infdis/135.5.800
DO - 10.1093/infdis/135.5.800
M3 - Article
C2 - 192812
AN - SCOPUS:0017656781
SN - 0022-1899
VL - 135
SP - 800
EP - 806
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -