Slow clearance of measles virus RNA after acute infection

Michaela A. Riddell, William J Moss, Debra Hauer, Mwaka Monze, Diane Griffin

Research output: Contribution to journalArticle

Abstract

Background: Measles virus (MV) RNA was detected 1 month after hospitalization with measles in more than half of Zambian children but the duration of detectable RNA was not determined. Objectives: To characterize the time course of MV clearance and identify factors associated with presence of viral RNA at late times after clinical recovery from infection. Study design: Blood, urine and nasopharyngeal specimens from 49 Zambian children with laboratory-confirmed measles were collected a median of 100 days (range 65-118) after rash onset. Samples were assayed for MV nucleocapsid and hemagglutinin RNA by reverse transcriptase-polymerase chain reaction. Amplified products were sequenced. Selected immunologic studies were performed. Results: MV RNA was detected in at least one specimen from 18 children (37%). Eighteen percent of 44 blood mononuclear cell, 23% of 30 nasopharyngeal and 50% of 6 urine specimens were positive. Detection was not associated with HIV-1 infection, % CD4+ T lymphocytes, plasma interleukin-10 levels or persistent MV-specific IgM. The MV genotype was D2 and sequences of late specimens were the same as specimens collected during acute illness. Conclusions: Presence of viral RNA at multiple sites more than 3 months after acute disease suggests that clearance of MV-infected cells occurs over many months.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalJournal of Clinical Virology
Volume39
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Measles virus
RNA
Infection
Viral RNA
Measles
Urine
Nucleocapsid
RNA-Directed DNA Polymerase
Hemagglutinins
Acute Disease
Exanthema
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-10
HIV Infections
Immunoglobulin M
HIV-1
Blood Cells
Hospitalization
Genotype
T-Lymphocytes

Keywords

  • Africa
  • HIV-1
  • Measles
  • RT-PCR
  • Virus clearance

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Slow clearance of measles virus RNA after acute infection. / Riddell, Michaela A.; Moss, William J; Hauer, Debra; Monze, Mwaka; Griffin, Diane.

In: Journal of Clinical Virology, Vol. 39, No. 4, 08.2007, p. 312-317.

Research output: Contribution to journalArticle

Riddell, Michaela A. ; Moss, William J ; Hauer, Debra ; Monze, Mwaka ; Griffin, Diane. / Slow clearance of measles virus RNA after acute infection. In: Journal of Clinical Virology. 2007 ; Vol. 39, No. 4. pp. 312-317.
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