Sleep drive is encoded by neural plastic changes in a dedicated circuit

Sha Liu, Qili Liu, Masashi Tabuchi, Mark N. Wu

Research output: Contribution to journalArticlepeer-review

135 Scopus citations


Prolonged wakefulness leads to an increased pressure for sleep, but how this homeostatic drive is generated and subsequently persists is unclear. Here, from a neural circuit screen in Drosophila, we identify a subset of ellipsoid body (EB) neurons whose activation generates sleep drive. Patch-clamp analysis indicates these EB neurons are highly sensitive to sleep loss, switching from spiking to burst-firing modes. Functional imaging and translational profiling experiments reveal that elevated sleep need triggers reversible increases in cytosolic Ca2+ levels, NMDA receptor expression, and structural markers of synaptic strength, suggesting these EB neurons undergo "sleep-need"-dependent plasticity. Strikingly, the synaptic plasticity of these EB neurons is both necessary and sufficient for generating sleep drive, indicating that sleep pressure is encoded by plastic changes within this circuit. These studies define an integrator circuit for sleep homeostasis and provide a mechanism explaining the generation and persistence of sleep drive.

Original languageEnglish (US)
Pages (from-to)1347-1360
Number of pages14
Issue number6
StatePublished - Jun 2 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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