Skin grafting facilitates the maintenance of head recording chambers for neurophysiological recording

Joong Ho Ahn, Chenkai Dai, Charles Coleman Della Santina

Research output: Contribution to journalArticle

Abstract

Maintaining a clean, quiescent tissue surface that is free of granulation and infection in the floor of a head-mounted chamber used for intracranial single-unit recording studies typically requires frequent cleaning. Considering the favourable outcomes of ontological surgical techniques that have long been used to create a dry, skin-lined mastoid cavity in patients with chronic otitis media, skin should be an ideal biological dressing to cover otherwise exposed dura mater in recording chambers. In chambers that required frequent cleaning, we harvested a thin layer of skin without hair follicles from the medial surface of the upper arms of two Rhesus monkeys and grafted the skin on the exposed dura surface. Each case resulted in a clean, dry, insensate, self-healing, easily maintained tissue surface that remained healthy despite the reduced frequency of chamber maintenance. We recommend this technique to reduce the potential for infection, to prevent cerebral spinal fluid leakage or bleeding in experiment and to minimise animal anxiety that might otherwise result from frequent chamber cleanings.

Original languageEnglish (US)
Pages (from-to)161-163
Number of pages3
JournalJournal of Neuroscience Methods
Volume215
Issue number2
DOIs
StatePublished - May 15 2013

Fingerprint

Skin Transplantation
Head
Maintenance
Skin
Biological Dressings
Dura Mater
Mastoid
Hair Follicle
Otitis Media
Infection
Macaca mulatta
Arm
Anxiety
Hemorrhage

Keywords

  • Intracranial recording
  • Monkey
  • Skin graft

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Skin grafting facilitates the maintenance of head recording chambers for neurophysiological recording. / Ahn, Joong Ho; Dai, Chenkai; Della Santina, Charles Coleman.

In: Journal of Neuroscience Methods, Vol. 215, No. 2, 15.05.2013, p. 161-163.

Research output: Contribution to journalArticle

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