Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting

Carolyn A. Meyers, Jiajia Xu, Leititia Zhang, Leslie Chang, Yiyun Wang, Greg Asatrian, Catherine Ding, Noah Yan, Erin Zou, Kristen Broderick, Min Lee, Bruno Peault, Aaron W. James

Research output: Contribution to journalArticle

Abstract

Human perivascular stem/stromal cells (PSC) are a multipotent mesenchymal progenitor cell population defined by their perivascular residence. PSC are increasingly studied for their application in skeletal regenerative medicine. PSC from subcutaneous white adipose tissue are most commonly isolated via fluorescence-activated cell sorting (FACS), and defined as a bipartite population of CD146+CD34-CD31-CD45- pericytes and CD34+CD146-CD31-CD45- adventitial cells. FACS poses several challenges for clinical translation, including requirements for facilities, equipment, and personnel. The purpose of this study is to identify if magnetic-activated cell sorting (MACS) is a feasible method to derive PSC, and to determine if MACS-derived PSC are comparable to our previous experience with FACS-derived PSC. In brief, CD146+ pericytes and CD34+ adventitial cells were enriched from human lipoaspirate using a multistep column approach. Next, cell identity and purity were analyzed by flow cytometry. In vitro multilineage differentiation studies were performed with MACS-defined PSC subsets. Finally, in vivo application was performed in nonhealing calvarial bone defects in Scid mice. Results showed that human CD146+ pericytes and CD34+ adventitial cells may be enriched by MACS, with defined purity, anticipated cell surface marker expression, and capacity for multilineage differentiation. In vivo, MACS-derived PSC induce ossification of bone defects. These data document the feasibility of a MACS approach for the enrichment and application of PSC in the field of tissue engineering and regenerative medicine.

Original languageEnglish (US)
Pages (from-to)1658-1666
Number of pages9
JournalTissue Engineering - Part A
Volume25
Issue number23-24
DOIs
StatePublished - Dec 2019

Fingerprint

Stem cells
Sorting
Stromal Cells
Stem Cells
Cells
Adventitia
Pericytes
Flow Cytometry
Fluorescence
Regenerative Medicine
Bone
Defects
Flow cytometry
Bone and Bones
White Adipose Tissue
Tissue engineering
Subcutaneous Fat
Tissue Engineering
Mesenchymal Stromal Cells
Osteogenesis

Keywords

  • bone regeneration
  • bone tissue engineering
  • MACS
  • magnetic activated cell sorting
  • mesenchymal stem cell
  • mesenchymal stromal cell

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting. / Meyers, Carolyn A.; Xu, Jiajia; Zhang, Leititia; Chang, Leslie; Wang, Yiyun; Asatrian, Greg; Ding, Catherine; Yan, Noah; Zou, Erin; Broderick, Kristen; Lee, Min; Peault, Bruno; James, Aaron W.

In: Tissue Engineering - Part A, Vol. 25, No. 23-24, 12.2019, p. 1658-1666.

Research output: Contribution to journalArticle

Meyers, CA, Xu, J, Zhang, L, Chang, L, Wang, Y, Asatrian, G, Ding, C, Yan, N, Zou, E, Broderick, K, Lee, M, Peault, B & James, AW 2019, 'Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting', Tissue Engineering - Part A, vol. 25, no. 23-24, pp. 1658-1666. https://doi.org/10.1089/ten.tea.2019.0031
Meyers, Carolyn A. ; Xu, Jiajia ; Zhang, Leititia ; Chang, Leslie ; Wang, Yiyun ; Asatrian, Greg ; Ding, Catherine ; Yan, Noah ; Zou, Erin ; Broderick, Kristen ; Lee, Min ; Peault, Bruno ; James, Aaron W. / Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting. In: Tissue Engineering - Part A. 2019 ; Vol. 25, No. 23-24. pp. 1658-1666.
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AU - Ding, Catherine

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