SIVQ-LCM protocol for the ArcturusXT instrument

Jason D. Hipp, Jerome Cheng, Jeffrey C. Hanson, Avi Z. Rosenberg, Michael R. Emmert-Buck, Michael A. Tangrea, Ulysses J. Balis

Research output: Contribution to journalArticlepeer-review

Abstract

SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.

Original languageEnglish (US)
Article numbere51662
JournalJournal of Visualized Experiments
Issue number89
DOIs
StatePublished - Jul 23 2014

Keywords

  • ArcturusXT
  • Bioengineering
  • Digital pathology
  • Image analysis
  • Issue 89
  • LCM
  • Personalized medicine
  • SIVQ

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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