TY - JOUR
T1 - Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin
AU - Del Rosario, Renato B.
AU - Wahl, Richard L.
PY - 1989
Y1 - 1989
N2 - Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.
AB - Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.
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U2 - 10.1016/0883-2897(89)90067-6
DO - 10.1016/0883-2897(89)90067-6
M3 - Article
C2 - 2807958
AN - SCOPUS:45249129898
SN - 0883-2897
VL - 16
SP - 525-527,529
JO - International Journal of Radiation Applications and Instrumentation.
JF - International Journal of Radiation Applications and Instrumentation.
IS - 5
ER -