Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

Renato B. Del Rosario, Richard L. Wahl

Research output: Contribution to journalArticle

Abstract

Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.

Original languageEnglish (US)
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume16
Issue number5
DOIs
StatePublished - 1989
Externally publishedYes

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Streptavidin
Biotin
Molecular Weight
Monoclonal Antibodies
Heterografts
Disulfides
Carcinoma
Antibodies

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Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin. / Del Rosario, Renato B.; Wahl, Richard L.

In: International Journal of Radiation Applications and Instrumentation., Vol. 16, No. 5, 1989.

Research output: Contribution to journalArticle

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