Site-specific gene expression in vivo by direct gene transfer into the arterial wall

Elizabeth G. Nabel, Gregory Plautz, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

A recombinant β-galactosidase gene has been expressed in a specific arterial segment in vivo by direct infection with a murine amphotropic retroviral vector or by DNA transfection with the use of liposomes. Several cell types in the vessel wall were transduced, including endothelial and vascular smooth muscle cells. After retroviral infection, a recombinant reporter gene was expressed for at least 5 months, and no helper virus was detected. Recombinant gene expression achieved by direct retroviral infection or liposome-mediated DNA transfection was limited to the site of infection and was absent from liver, lung, kidney, and spleen. These results demonstrate that site-specific gene expression can be achieved by direct gene transfer in vivo and could be applied to the treatment of such human diseases as atherosclerosis or cancer.

Original languageEnglish (US)
Pages (from-to)1285-1288
Number of pages4
JournalScience
Volume249
Issue number4974
StatePublished - Sep 14 1990
Externally publishedYes

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Gene Expression
Infection
Liposomes
Genes
Transfection
Galactosidases
Helper Viruses
DNA
Reporter Genes
Vascular Smooth Muscle
Smooth Muscle Myocytes
Atherosclerosis
Spleen
Kidney
Lung
Liver
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Nabel, E. G., Plautz, G., & Nabel, G. J. (1990). Site-specific gene expression in vivo by direct gene transfer into the arterial wall. Science, 249(4974), 1285-1288.

Site-specific gene expression in vivo by direct gene transfer into the arterial wall. / Nabel, Elizabeth G.; Plautz, Gregory; Nabel, Gary J.

In: Science, Vol. 249, No. 4974, 14.09.1990, p. 1285-1288.

Research output: Contribution to journalArticle

Nabel, EG, Plautz, G & Nabel, GJ 1990, 'Site-specific gene expression in vivo by direct gene transfer into the arterial wall', Science, vol. 249, no. 4974, pp. 1285-1288.
Nabel EG, Plautz G, Nabel GJ. Site-specific gene expression in vivo by direct gene transfer into the arterial wall. Science. 1990 Sep 14;249(4974):1285-1288.
Nabel, Elizabeth G. ; Plautz, Gregory ; Nabel, Gary J. / Site-specific gene expression in vivo by direct gene transfer into the arterial wall. In: Science. 1990 ; Vol. 249, No. 4974. pp. 1285-1288.
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