Site-specific fluorescence spectrum detection and characterization of hASIC1a channels upon toxin mambalgin-1 binding in live mammalian cells

Ming Wen, Xiaoqi Guo, Peibei Sun, Liang Xiao, Juan Li, Ying Xiong, Jin Bao, Tian Xue, Longhua Zhang, Changlin Tian

Research output: Contribution to journalArticle

Abstract

The synthesis of fluorescent unnatural amino-acid Anap was optimized and the Anap was incorporated into four sites in an acid-pocket or a transmembrane region of human acid-sensing ion channel-1a (hASIC1a). Combinational Anap fluorescence spectra and patch-clamp electrophysiology data illustrated site-specific conformational responses upon toxin mambalgin-1 binding. This combinational approach can be used to analyse conformational properties of many different eukaryotic proteins in their functional states, in a site-specific manner in live mammalian cells.

Original languageEnglish (US)
Pages (from-to)8153-8156
Number of pages4
JournalChemical Communications
Volume51
Issue number38
DOIs
StatePublished - May 11 2015
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys
  • Medicine(all)

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