Site-directed mutagenesis of conserved aspartates, glutamates and arginines in the active site region of Escherichia coli DNA topoisomerase I

Medicine & Life Sciences

• arginine glutamate 100%
• arginine aspartate 98%
• Type I DNA Topoisomerase 77%
• Site-Directed Mutagenesis 63%
• Catalytic Domain 53%
• Escherichia coli 41%
• DNA 32%
• DNA Cleavage 31%
• Hydroxyl Radical 26%
• Mutation 19%
• DNA Topoisomerases 19%
• Enzymes 19%
• Acidic Amino Acids 17%
• Superhelical DNA 17%
• Basic Amino Acids 16%
• Type II DNA Topoisomerase 15%
• Phosphotyrosine 15%
• Catalysis 14%
• Alanine 12%
• Tyrosine 10%
• In Vitro Techniques 5%

Chemical Compounds

• Aspartate 65%
• Site-Directed Mutagenesis 61%
• Arginine 59%
• Mutation 49%
• Cleavage 26%
• Alanine 18%
• Tyrosine 17%
• Catalysis 12%
• Crystal Structure 9%
• Acid 6%