Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia

Samuel S. Chong, Corinne D. Boehm, Douglas R. Higgs, Garry R. Cutting

Research output: Contribution to journalArticlepeer-review

395 Scopus citations

Abstract

α-Thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalls). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of α-thalassemia. The assay allows simple, high throughput genetic screening for these common hematological disorders.

Original languageEnglish (US)
Pages (from-to)360-362
Number of pages3
JournalBlood
Volume95
Issue number1
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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