Single 19F probe for simultaneous detection of multiple metal ions using miCEST MRI

Amnon Bar-Shir, Nirbhay N. Yadav, Assaf A. Gilad, Peter C.M. Van Zijl, Michael T. McMahon, Jeff W.M. Bulte

Research output: Contribution to journalArticle

Abstract

The local presence and concentration of metal ions in biological systems has been extensively studied ex vivo using fluorescent dyes. However, the detection of multiple metal ions in vivo remains a major challenge. We present a magnetic resonance imaging (MRI)-based method for noninvasive detection of specific ions that may be coexisting, using the tetrafluorinated derivative of the BAPTA (TF-BAPTA) chelate as a 19F chelate analogue of existing optical dyes. Taking advantage of the difference in the ion-specific 19F nuclear magnetic resonance (NMR) chemical shift offset (δ ω) values between the ion-bound and free TF-BAPTA, we exploited the dynamic exchange between ion-bound and free TF-BAPTA to obtain MRI contrast with multi-ion chemical exchange saturation transfer (miCEST). We demonstrate that TF-BAPTA as a prototype single 19F probe can be used to separately visualize mixed Zn2+ and Fe2+ ions in a specific and simultaneous fashion, without interference from potential competitive ions.

Original languageEnglish (US)
Pages (from-to)78-81
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number1
DOIs
StatePublished - Jan 14 2015

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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