TY - JOUR
T1 - Single-Pair and Small-Group Crosses of Aedes aegypti
AU - Wohl, Margot P.
AU - McMeniman, Conor J.
N1 - Funding Information:
We acknowledge generous funding to C.J.M. from the National Institutes of Health (R21 AI146450-01), Innovative Vector Control Consortium, Johns Hopkins Malaria Research Institute, and Bloom-berg Philanthropies. M.P.W. is supported by a postdoctoral fellowship under The Molecular and Cellular Basis of Infectious Diseases (MCBID) Program (T32A1007417) from the National Institutes of Health.
Funding Information:
We acknowledge generous funding to C.J.M. from the National Institutes of Health (R21 AI146450-01), Innovative Vector Control Consortium, Johns Hopkins Malaria Research Institute, and Bloomberg Philanthropies. M.P.W. is supported by a postdoctoral fellowship under The Molecular and Cellular Basis of Infectious Diseases (MCBID) Program (T32A1007417) from the National Institutes of Health.
Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.
PY - 2023/3
Y1 - 2023/3
N2 - Aedes aegypti is an emerging model insect species used in genetics studies because of its ease of laboratory rearing, desiccation-resistant eggs, expanding genetic toolkit, and high-quality reference genome. Here, we describe procedures to isolate and sex virgin female and male mosquitoes and establish successful mating crosses. We also detail how to blood feed mosquitoes from these crosses, isolate individual or small groups of females for egg laying, condition these eggs for storage and hatching, and verify female mating status.
AB - Aedes aegypti is an emerging model insect species used in genetics studies because of its ease of laboratory rearing, desiccation-resistant eggs, expanding genetic toolkit, and high-quality reference genome. Here, we describe procedures to isolate and sex virgin female and male mosquitoes and establish successful mating crosses. We also detail how to blood feed mosquitoes from these crosses, isolate individual or small groups of females for egg laying, condition these eggs for storage and hatching, and verify female mating status.
UR - http://www.scopus.com/inward/record.url?scp=85149173950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85149173950&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot108018
DO - 10.1101/pdb.prot108018
M3 - Article
C2 - 36223993
AN - SCOPUS:85149173950
SN - 1940-3402
VL - 2023
SP - 186
EP - 193
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 3
ER -