Rapid positioning of single molecules in the excitation volume of a confocal (or near-field) microscope is necessary for the successful spectroscopy of molecules with fluctuating emission properties. A computer controlled optical system which automatically and rapidly locates and performs spectroscopic measurements on single molecules was developed. Examples for such spectroscopies, performed on short-lived molecules, are given.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)