Single-molecule FRET analysis of helicase functions

Eli Rothenberg, Taekjip Ha

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.

Original languageEnglish (US)
Title of host publicationHelicases: Methods and Protocols
Pages29-43
Number of pages15
Volume587
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume587
ISSN (Print)10643745

Fingerprint

Fluorescence Resonance Energy Transfer
Enzymes

Keywords

  • binding
  • DNA
  • FRET
  • Helicase
  • single molecule
  • translocation
  • unwinding

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Rothenberg, E., & Ha, T. (2010). Single-molecule FRET analysis of helicase functions. In Helicases: Methods and Protocols (Vol. 587, pp. 29-43). (Methods in Molecular Biology; Vol. 587). https://doi.org/10.1007/978-1-60327-355-8_3

Single-molecule FRET analysis of helicase functions. / Rothenberg, Eli; Ha, Taekjip.

Helicases: Methods and Protocols. Vol. 587 2010. p. 29-43 (Methods in Molecular Biology; Vol. 587).

Research output: Chapter in Book/Report/Conference proceedingChapter

Rothenberg, E & Ha, T 2010, Single-molecule FRET analysis of helicase functions. in Helicases: Methods and Protocols. vol. 587, Methods in Molecular Biology, vol. 587, pp. 29-43. https://doi.org/10.1007/978-1-60327-355-8_3
Rothenberg E, Ha T. Single-molecule FRET analysis of helicase functions. In Helicases: Methods and Protocols. Vol. 587. 2010. p. 29-43. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-355-8_3
Rothenberg, Eli ; Ha, Taekjip. / Single-molecule FRET analysis of helicase functions. Helicases: Methods and Protocols. Vol. 587 2010. pp. 29-43 (Methods in Molecular Biology).
@inbook{ada96a4d626b4c058e4c1f639b03d47a,
title = "Single-molecule FRET analysis of helicase functions",
abstract = "In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.",
keywords = "binding, DNA, FRET, Helicase, single molecule, translocation, unwinding",
author = "Eli Rothenberg and Taekjip Ha",
year = "2010",
doi = "10.1007/978-1-60327-355-8_3",
language = "English (US)",
isbn = "9781603273541",
volume = "587",
series = "Methods in Molecular Biology",
pages = "29--43",
booktitle = "Helicases: Methods and Protocols",

}

TY - CHAP

T1 - Single-molecule FRET analysis of helicase functions

AU - Rothenberg, Eli

AU - Ha, Taekjip

PY - 2010

Y1 - 2010

N2 - In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.

AB - In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.

KW - binding

KW - DNA

KW - FRET

KW - Helicase

KW - single molecule

KW - translocation

KW - unwinding

UR - http://www.scopus.com/inward/record.url?scp=77952992155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952992155&partnerID=8YFLogxK

U2 - 10.1007/978-1-60327-355-8_3

DO - 10.1007/978-1-60327-355-8_3

M3 - Chapter

C2 - 20225140

AN - SCOPUS:77952992155

SN - 9781603273541

VL - 587

T3 - Methods in Molecular Biology

SP - 29

EP - 43

BT - Helicases: Methods and Protocols

ER -