Single-molecule four-color FRET

Jinwoo Lee, Sanghwa Lee, Kaushik Ragunathan, Chirlmin Joo, Taekjip Ha, Sungchul Hohng

Research output: Contribution to journalArticle

Abstract

Six interfluorophore FRET efficiencies Eij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

Original languageEnglish (US)
Pages (from-to)9922-9925
Number of pages4
JournalAngewandte Chemie - International Edition
Volume49
Issue number51
DOIs
StatePublished - Dec 17 2010
Externally publishedYes

Keywords

  • DNA structures
  • FRET
  • conformational dynamics
  • fluorescence
  • single-molecule studies

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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  • Cite this

    Lee, J., Lee, S., Ragunathan, K., Joo, C., Ha, T., & Hohng, S. (2010). Single-molecule four-color FRET. Angewandte Chemie - International Edition, 49(51), 9922-9925. https://doi.org/10.1002/anie.201005402