TY - JOUR
T1 - Single-molecule fluorescence microscopy of native macromolecular complexes
AU - Aggarwal, Vasudha
AU - Ha, Taekjip
N1 - Funding Information:
We thank Benjamin Leslie, Anustup Poddar and Olivia Yang for their comments and proof-reading the review. T.H. is funded by the Howard Hughes Medical Institute .
Publisher Copyright:
© 2016 Elsevier Ltd
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.
AB - Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.
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U2 - 10.1016/j.sbi.2016.09.006
DO - 10.1016/j.sbi.2016.09.006
M3 - Review article
C2 - 27662375
AN - SCOPUS:84988370282
SN - 0959-440X
VL - 41
SP - 225
EP - 232
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -