TY - JOUR
T1 - Single-molecule fluorescence methods for the study of nucleic acids
AU - Ha, T.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001/6/1
Y1 - 2001/6/1
N2 - Single-molecule fluorescence methods and biomechanical tools provide exciting new opportunities to probe biochemical processes in unprecedented detail. The detection and spectroscopy of single fluorophores have recently been used to observe conformational changes and biochemical events involving nucleic acids. A number of fluorescence observables, including localization, quenching, polarization response and fluorescence resonance energy transfer, have been utilized. An exciting new opportunity of combining fluorescence methods and biomechanical tools to study the structural changes and functions of enzymes that participate in nucleic acid metabolism has also arisen.
AB - Single-molecule fluorescence methods and biomechanical tools provide exciting new opportunities to probe biochemical processes in unprecedented detail. The detection and spectroscopy of single fluorophores have recently been used to observe conformational changes and biochemical events involving nucleic acids. A number of fluorescence observables, including localization, quenching, polarization response and fluorescence resonance energy transfer, have been utilized. An exciting new opportunity of combining fluorescence methods and biomechanical tools to study the structural changes and functions of enzymes that participate in nucleic acid metabolism has also arisen.
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U2 - 10.1016/S0959-440X(00)00204-9
DO - 10.1016/S0959-440X(00)00204-9
M3 - Review article
C2 - 11406376
AN - SCOPUS:0035366378
SN - 0959-440X
VL - 11
SP - 287
EP - 292
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 3
ER -