Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit

Pravin S. Shirude, Burak Okumus, Liming Ying, Taekjip Ha, Shankar Balasubramanian

Research output: Contribution to journalArticle

Abstract

Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.

Original languageEnglish (US)
Pages (from-to)7484-7485
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number24
DOIs
StatePublished - Jun 20 2007
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit'. Together they form a unique fingerprint.

  • Cite this