Single-molecule analysis of chemotactic signaling in Dictyostelium cells

M. Ueda, Y. Sako, T. Tanaka, P. Devreotes, T. Yanagida

Research output: Contribution to journalArticle

Abstract

Single-molecule imaging techniques were used to reveal the binding of individual cyclic adenosine 3′,5′-monophosphate molecules to heterotrimeric guanine nucleotide-binding protein coupled receptors on the surface of living Dictyostelium discoideum cells. The binding sites were uniformly distributed and diffused rapidly in the plane of the membrane. The probabilities of individual association and dissociation events were greater for receptors at the anterior end of the cell. Agonist-induced receptor phosphorylation had little effect on any of the monitored properties, whereas G protein coupling influenced the binding kinetics. These observations illustrate the dynamic properties of receptors involved in gradient sensing and suggest that these may be polarized in chemotactic cells.

Original languageEnglish (US)
Pages (from-to)864-867
Number of pages4
JournalScience
Volume294
Issue number5543
DOIs
StatePublished - Oct 26 2001

ASJC Scopus subject areas

  • General

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