Single H2Kb, H2Db and double H2KbDb knockout mice: Peripheral CD8+ T cell repertoire and antilymphocytic choriomeningitis virus cytolytic responses

Béatrice Pérarnau, Marie Françoise Saron, Bernardo Reina San Martin, Nathalie Bervas, Helena Ong, Mark J. Soloski, Austin G. Smith, Jan M. Ure, Jean Edouard Gairin, François A. Lemonnier

Research output: Contribution to journalArticle

Abstract

Single H2Kb, H2Db and double H2KbDb homozygous knockout (KO) mice were generated and their peripheral CD8+ T cell repertoires compared to that of C57BL/6 (B6) mice. Limited (10-20%, H2Db), substantial (30-50%, H2Kb) and profound (90%, H2KbDb) reduction of peripheral CD8+ T cells was observed in KO mice, without Vβ diversity alteration. Classical class Ia molecules therefore ensure most but not all of the peripheral CD8+ T cell repertoire education. As expected, H2Kb but also H2Db KO mice developed choriomeningitis following intracranial infection by lymphocytic choriomeningitis virus with the same kinetics, lethality and CD8+ cell implication as wild-type B6 mice. By contrast, H2KbDb (class Ia-Ib+) KO mice survived. Choriomeningitis of H2Db KO mice was linked to the development of a subdominant (in normal B6 mice) H2Kb-restricted cytotoxic T lymphocyte response. Mice expressing a restricted set of histocompatibility class I molecules should represent useful tools to evaluate the immunological potentials of individual MHC class I molecules.

Original languageEnglish (US)
Pages (from-to)1243-1252
Number of pages10
JournalEuropean Journal of Immunology
Volume29
Issue number4
DOIs
StatePublished - Jan 1 1999

Keywords

  • Cytotoxic T lymphocyte
  • Gene targeting
  • Histocompatibility antigen class I
  • Lymphocytic choriomeningitis
  • MHC

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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