TY - JOUR
T1 - Single H2Kb, H2Db and double H2KbDb knockout mice
T2 - Peripheral CD8+ T cell repertoire and antilymphocytic choriomeningitis virus cytolytic responses
AU - Pérarnau, Béatrice
AU - Saron, Marie Françoise
AU - Reina San Martin, Bernardo
AU - Bervas, Nathalie
AU - Ong, Helena
AU - Soloski, Mark J.
AU - Smith, Austin G.
AU - Ure, Jan M.
AU - Gairin, Jean Edouard
AU - Lemonnier, François A.
PY - 1999
Y1 - 1999
N2 - Single H2Kb, H2Db and double H2KbDb homozygous knockout (KO) mice were generated and their peripheral CD8+ T cell repertoires compared to that of C57BL/6 (B6) mice. Limited (10-20%, H2Db), substantial (30-50%, H2Kb) and profound (90%, H2KbDb) reduction of peripheral CD8+ T cells was observed in KO mice, without Vβ diversity alteration. Classical class Ia molecules therefore ensure most but not all of the peripheral CD8+ T cell repertoire education. As expected, H2Kb but also H2Db KO mice developed choriomeningitis following intracranial infection by lymphocytic choriomeningitis virus with the same kinetics, lethality and CD8+ cell implication as wild-type B6 mice. By contrast, H2KbDb (class Ia-Ib+) KO mice survived. Choriomeningitis of H2Db KO mice was linked to the development of a subdominant (in normal B6 mice) H2Kb-restricted cytotoxic T lymphocyte response. Mice expressing a restricted set of histocompatibility class I molecules should represent useful tools to evaluate the immunological potentials of individual MHC class I molecules.
AB - Single H2Kb, H2Db and double H2KbDb homozygous knockout (KO) mice were generated and their peripheral CD8+ T cell repertoires compared to that of C57BL/6 (B6) mice. Limited (10-20%, H2Db), substantial (30-50%, H2Kb) and profound (90%, H2KbDb) reduction of peripheral CD8+ T cells was observed in KO mice, without Vβ diversity alteration. Classical class Ia molecules therefore ensure most but not all of the peripheral CD8+ T cell repertoire education. As expected, H2Kb but also H2Db KO mice developed choriomeningitis following intracranial infection by lymphocytic choriomeningitis virus with the same kinetics, lethality and CD8+ cell implication as wild-type B6 mice. By contrast, H2KbDb (class Ia-Ib+) KO mice survived. Choriomeningitis of H2Db KO mice was linked to the development of a subdominant (in normal B6 mice) H2Kb-restricted cytotoxic T lymphocyte response. Mice expressing a restricted set of histocompatibility class I molecules should represent useful tools to evaluate the immunological potentials of individual MHC class I molecules.
KW - Cytotoxic T lymphocyte
KW - Gene targeting
KW - Histocompatibility antigen class I
KW - Lymphocytic choriomeningitis
KW - MHC
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U2 - 10.1002/(SICI)1521-4141(199904)29:04<1243::AID-IMMU1243>3.0.CO;2-A
DO - 10.1002/(SICI)1521-4141(199904)29:04<1243::AID-IMMU1243>3.0.CO;2-A
M3 - Article
C2 - 10229092
AN - SCOPUS:0032587063
SN - 0014-2980
VL - 29
SP - 1243
EP - 1252
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -