@inbook{33e2f962af014b679977c760adfb53ca,
title = "Single-cell analysis of T-cell receptor αβ repertoire",
abstract = "The unbiased, paired analysis of T-cell receptor (TCR) α- and β-chain usage at the single-cell level provides a valuable window of understanding into the TCR repertoire and the nature of the immune response. Earlier technologies for TCR repertoire analysis were often limited to examining TCR complementarity determining region 3 (CDR3) β expression or required in vitro cloning procedures that can artificially skew the TCR repertoire from its in vivo state. We describe here a direct ex vivo, single-cell-based strategy for the clonotypic analysis of TCRαβ repertoires that utilizes multiplexed panels of TCRα and TCRβ- specific primers in a nested PCR to amplify expressed transcripts from individual, epitope-specific T cells. This strategy yields the paired TCRαβ sequences of any given population of αβ T cells of interest.",
keywords = "Clonotype, Complementarity-determining region 3, Epitope, Paired analysis, Single-cell analysis, T-cell receptor repertoire, TCR alpha, TCR beta, TCR diversity, Unbiased repertoire",
author = "Pradyot Dash and Wang, {George C.} and Thomas, {Paul G.}",
note = "Publisher Copyright: {\textcopyright} Springer Science Business Media New York 2015.",
year = "2015",
doi = "10.1007/978-1-4939-2963-4_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "181--197",
booktitle = "Methods in Molecular Biology",
}