TY - JOUR
T1 - Single-cell analysis of G-protein signal transduction
AU - Clister, Terri
AU - Mehta, Sohum
AU - Zhang, Jin
PY - 2015/3/13
Y1 - 2015/3/13
N2 - The growing use of fluorescent biosensors to directly probe the spatiotemporal dynamics of biochemical processes in living cells has revolutionized the study of intracellular signaling. In this review, we summarize recent developments in the use of biosensors to illuminate the molecular details of G-protein-coupled receptor (GPCR) signaling pathways, which have long served as the model for our understanding of signal transduction, while also offering our perspectives on the future of this exciting field. Specifically, we highlight several ways in which biosensor-based single-cell analyses are being used to unravel many of the enduring mysteries that surround these diverse signaling pathways.
AB - The growing use of fluorescent biosensors to directly probe the spatiotemporal dynamics of biochemical processes in living cells has revolutionized the study of intracellular signaling. In this review, we summarize recent developments in the use of biosensors to illuminate the molecular details of G-protein-coupled receptor (GPCR) signaling pathways, which have long served as the model for our understanding of signal transduction, while also offering our perspectives on the future of this exciting field. Specifically, we highlight several ways in which biosensor-based single-cell analyses are being used to unravel many of the enduring mysteries that surround these diverse signaling pathways.
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U2 - 10.1074/jbc.R114.616391
DO - 10.1074/jbc.R114.616391
M3 - Article
C2 - 25605723
AN - SCOPUS:84924872556
SN - 0021-9258
VL - 290
SP - 6681
EP - 6688
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 11
ER -