Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic: IFNB1 expression

Sultan Doǧanay, Maurice Youzong Lee, Alina Baum, Jessie Peh, Sun Young Hwang, Joo Yeon Yoo, Paul J. Hergenrother, Adolfo García-Sastre, Sua Myong, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

RIG-I-like receptors (RLRs) are cytoplasmic sensors of viral RNA that trigger the signaling cascade that leads to type I interferon (IFN) production. Transcriptional induction of RLRs by IFN is believed to play the role of positive feedback to further amplify viral sensing. We found that RLRs and several other IFN-stimulated genes (ISGs) are induced early in viral infection independent of IFN. Expression of these early ISGs requires IRF3/IRF7 and is highly correlated amongst them. Simultaneous detection of mRNA of IFNB1, viral replicase, and ISGs revealed distinct populations of IFNB1 expressing and non-expressing cells which are highly correlated with the levels of early ISGs but are uncorrelated with IFN-dependent ISGs and viral gene expression. Individual expression of RLRs made IFNB1 expression more robust and earlier, suggesting a causal relation between levels of RLR and induction of IFN.

Original languageEnglish (US)
Pages (from-to)857-867
Number of pages11
JournalIntegrative Biology (United Kingdom)
Volume9
Issue number11
DOIs
StatePublished - Nov 1 2017

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Single-Cell Analysis
Gene expression
Antiviral Agents
Genes
Interferons
Gene Expression
Interferon Receptors
Interferon Type I
Viral Genes
Viral RNA
Virus Diseases
Cytoplasmic and Nuclear Receptors
Feedback
Messenger RNA
Sensors
Population

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic : IFNB1 expression. / Doǧanay, Sultan; Lee, Maurice Youzong; Baum, Alina; Peh, Jessie; Hwang, Sun Young; Yoo, Joo Yeon; Hergenrother, Paul J.; García-Sastre, Adolfo; Myong, Sua; Ha, Taekjip.

In: Integrative Biology (United Kingdom), Vol. 9, No. 11, 01.11.2017, p. 857-867.

Research output: Contribution to journalArticle

Doǧanay, S, Lee, MY, Baum, A, Peh, J, Hwang, SY, Yoo, JY, Hergenrother, PJ, García-Sastre, A, Myong, S & Ha, T 2017, 'Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic: IFNB1 expression', Integrative Biology (United Kingdom), vol. 9, no. 11, pp. 857-867. https://doi.org/10.1039/c7ib00146k
Doǧanay, Sultan ; Lee, Maurice Youzong ; Baum, Alina ; Peh, Jessie ; Hwang, Sun Young ; Yoo, Joo Yeon ; Hergenrother, Paul J. ; García-Sastre, Adolfo ; Myong, Sua ; Ha, Taekjip. / Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic : IFNB1 expression. In: Integrative Biology (United Kingdom). 2017 ; Vol. 9, No. 11. pp. 857-867.
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