Simultanous determination of dacarbazine, its photolytic degradation product, 2-azahypoxanthine, and the metabolite 5-aminoimidazole-4-carboxamide in plasma and urine by high-pressure liquid chromatography

D. Fiore, A. J. Jackson, M. S. Didolkar, V. R. Dandu

Research output: Contribution to journalArticle

Abstract

A high-pressure liquid chromatographic method has been developed that allows for the simultaneous analysis of dacarbazine (DTIC), 5-aminoimidazole-4-carboxamide (AIC), and 2-azahypoxanthine (2-AZA) in plasma or urine. Plasma samples were prepared by ultrafiltration, whereas urine samples were filtered and diluted for analysis. Chromatography was done with a C18 μBondapak column along with gradient elution of the drugs. The mobile phase consisted of 100% 0.5 M sodium acetate (pH 7.0) and 25% acetonitrile in 0.05 M sodium acetate (pH 5.5) with detection at 280 nm. Linearity was observed up to 500 μg/ml for DTIC and up to 53 μg/ml for AIC and 2-AZA. The assay methodology was reproducible, with a lower limit detection of 5.0, 0.5, and 0.5 μg/ml for DTIC, AIC, and 2-AZA, respectively. Interday and intraday coefficients of variation ranged between 4 to 14% and 2 to 16%, respectively. The analytical method was applied to the analysis of plasma and urine samples resulting from the isolation perfusion chemotherapy of an extremity with 57 mg of DTIC per kg in a patient with melanoma.

Original languageEnglish (US)
Pages (from-to)977-979
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume27
Issue number6
StatePublished - 1985
Externally publishedYes

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Aminoimidazole Carboxamide
Dacarbazine
High Pressure Liquid Chromatography
Urine
Sodium Acetate
Ultrafiltration
Limit of Detection
Chromatography
Melanoma
Extremities
Perfusion
2-azahypoxanthine
4-aminoimidazole
Pressure
Drug Therapy
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

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title = "Simultanous determination of dacarbazine, its photolytic degradation product, 2-azahypoxanthine, and the metabolite 5-aminoimidazole-4-carboxamide in plasma and urine by high-pressure liquid chromatography",
abstract = "A high-pressure liquid chromatographic method has been developed that allows for the simultaneous analysis of dacarbazine (DTIC), 5-aminoimidazole-4-carboxamide (AIC), and 2-azahypoxanthine (2-AZA) in plasma or urine. Plasma samples were prepared by ultrafiltration, whereas urine samples were filtered and diluted for analysis. Chromatography was done with a C18 μBondapak column along with gradient elution of the drugs. The mobile phase consisted of 100{\%} 0.5 M sodium acetate (pH 7.0) and 25{\%} acetonitrile in 0.05 M sodium acetate (pH 5.5) with detection at 280 nm. Linearity was observed up to 500 μg/ml for DTIC and up to 53 μg/ml for AIC and 2-AZA. The assay methodology was reproducible, with a lower limit detection of 5.0, 0.5, and 0.5 μg/ml for DTIC, AIC, and 2-AZA, respectively. Interday and intraday coefficients of variation ranged between 4 to 14{\%} and 2 to 16{\%}, respectively. The analytical method was applied to the analysis of plasma and urine samples resulting from the isolation perfusion chemotherapy of an extremity with 57 mg of DTIC per kg in a patient with melanoma.",
author = "D. Fiore and Jackson, {A. J.} and Didolkar, {M. S.} and Dandu, {V. R.}",
year = "1985",
language = "English (US)",
volume = "27",
pages = "977--979",
journal = "Antimicrobial Agents and Chemotherapy",
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TY - JOUR

T1 - Simultanous determination of dacarbazine, its photolytic degradation product, 2-azahypoxanthine, and the metabolite 5-aminoimidazole-4-carboxamide in plasma and urine by high-pressure liquid chromatography

AU - Fiore, D.

AU - Jackson, A. J.

AU - Didolkar, M. S.

AU - Dandu, V. R.

PY - 1985

Y1 - 1985

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