Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism

Li Hui Tseng; Pei Jer Chen; Ming Tseh Lin; Kentner Singleton; Emily G. Martin; Ai Hsin Yen; Paul J. Martin; John A. Hansen

doi:10.1016/S0022-1759(02)00165-5

Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism

Li Hui Tseng, Pei Jer Chen, Ming Tseh Lin, Kentner Singleton, Emily G. Martin, Ai Hsin Yen, Paul J. Martin, John A. Hansen

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex.

Original language	English (US)
Pages (from-to)	151-156
Number of pages	6
Journal	Journal of Immunological Methods
Volume	267
Issue number	2
DOIs	https://doi.org/10.1016/S0022-1759(02)00165-5
State	Published - Sep 15 2002
Externally published	Yes

Keywords

IL-1
Multiplex PCR
Polymorphism
RFLP

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0022-1759(02)00165-5

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Tseng, L. H., Chen, P. J., Lin, M. T., Singleton, K., Martin, E. G., Yen, A. H., Martin, P. J., & Hansen, J. A. (2002). Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism. Journal of Immunological Methods, 267(2), 151-156. https://doi.org/10.1016/S0022-1759(02)00165-5

Tseng, LH, Chen, PJ, Lin, MT, Singleton, K, Martin, EG, Yen, AH, Martin, PJ & Hansen, JA 2002, 'Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism', Journal of Immunological Methods, vol. 267, no. 2, pp. 151-156. https://doi.org/10.1016/S0022-1759(02)00165-5

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title = "Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism",

abstract = "The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex.",

keywords = "IL-1, Multiplex PCR, Polymorphism, RFLP",

author = "Tseng, {Li Hui} and Chen, {Pei Jer} and Lin, {Ming Tseh} and Kentner Singleton and Martin, {Emily G.} and Yen, {Ai Hsin} and Martin, {Paul J.} and Hansen, {John A.}",

note = "Funding Information: DNA samples used in this study were obtained from the IHWG cytokine gene polymorphism reference panel supported by U24 AI49213. This work was supported in part by Grants AI33484, AI49213, CA15704 and CA18029 from the National Institutes of Health, USA; NHRI-EX90-8829SP from National Health Research Institute, Taiwan; and NTUH89F007 from National Taiwan University Hospital, Taiwan.",

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doi = "10.1016/S0022-1759(02)00165-5",

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AU - Tseng, Li Hui

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AU - Lin, Ming Tseh

AU - Singleton, Kentner

AU - Martin, Emily G.

AU - Yen, Ai Hsin

AU - Martin, Paul J.

AU - Hansen, John A.

N1 - Funding Information: DNA samples used in this study were obtained from the IHWG cytokine gene polymorphism reference panel supported by U24 AI49213. This work was supported in part by Grants AI33484, AI49213, CA15704 and CA18029 from the National Institutes of Health, USA; NHRI-EX90-8829SP from National Health Research Institute, Taiwan; and NTUH89F007 from National Taiwan University Hospital, Taiwan.

PY - 2002/9/15

Y1 - 2002/9/15

N2 - The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex.

AB - The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex.

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Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism

Abstract

Keywords

ASJC Scopus subject areas

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