Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism

Li Hui Tseng, Pei Jer Chen, Ming Tseh Lin, Kentner Singleton, Emily G. Martin, Ai Hsin Yen, Paul J. Martin, John A. Hansen

Research output: Contribution to journalArticlepeer-review

Abstract

The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalJournal of Immunological Methods
Volume267
Issue number2
DOIs
StatePublished - Sep 15 2002
Externally publishedYes

Keywords

  • IL-1
  • Multiplex PCR
  • Polymorphism
  • RFLP

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism'. Together they form a unique fingerprint.

Cite this