Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization

Edward J. Cone, William Buchwald, David Yousefnejad

Research output: Contribution to journalArticle

Abstract

Phencyclidine and monohydroxy metabolites were measured in human urine using gas chromatography-mass fragmentography with methane chemical ionization. Samples were extracted either untreated or following acid hydrolysis, derivatized with heptafluorobutyric anhydride, separated on a 3% SE-30 column and analyzed by mass fragmentography. The assay was sensitive to ca. 0.01 μg/ml for phencyclidine and ca. 0.05 μg/ml for the metabolites. Urine samples from five human subjects enrolled in a methadone maintenance program who had ingested phencyclidine were analyzed. The phencyclidine concentration ranged from 0.3 to 23.7 μg/ml. The concentrations of metabolites ranged from 0 to 1.8 μg/ml. A new monohydroxy metabolite was detected in the samples, but its structure was not fully elucidated. The specificity of the assay was examined.

Original languageEnglish (US)
Pages (from-to)331-339
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume223
Issue number2
DOIs
StatePublished - May 8 1981
Externally publishedYes

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Phencyclidine
Methane
Metabolites
Gas chromatography
Gas Chromatography
Ionization
Urine
heptafluorobutyric anhydride
Assays
Silicone Elastomers
Methadone
Hydrolysis
Acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization. / Cone, Edward J.; Buchwald, William; Yousefnejad, David.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 223, No. 2, 08.05.1981, p. 331-339.

Research output: Contribution to journalArticle

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