Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization

Edward J. Cone, William Buchwald, David Yousefnejad

Research output: Contribution to journalArticlepeer-review

Abstract

Phencyclidine and monohydroxy metabolites were measured in human urine using gas chromatography-mass fragmentography with methane chemical ionization. Samples were extracted either untreated or following acid hydrolysis, derivatized with heptafluorobutyric anhydride, separated on a 3% SE-30 column and analyzed by mass fragmentography. The assay was sensitive to ca. 0.01 μg/ml for phencyclidine and ca. 0.05 μg/ml for the metabolites. Urine samples from five human subjects enrolled in a methadone maintenance program who had ingested phencyclidine were analyzed. The phencyclidine concentration ranged from 0.3 to 23.7 μg/ml. The concentrations of metabolites ranged from 0 to 1.8 μg/ml. A new monohydroxy metabolite was detected in the samples, but its structure was not fully elucidated. The specificity of the assay was examined.

Original languageEnglish (US)
Pages (from-to)331-339
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume223
Issue number2
DOIs
StatePublished - May 8 1981

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization'. Together they form a unique fingerprint.

Cite this