Simultaneous arming and structure/activity studies of natural products employing O-H insertions: An expedient and versatile strategy for natural products-based chemical genetics

Satyamaheshwar Peddibhotla, Yongjun Dang, Jun O. Liu, Daniel Romo

Research output: Contribution to journalArticlepeer-review

Abstract

The identification of "druggable" targets is an immediate opportunity and challenge in the post-genomic era. Natural products are enduring tools for basic cellular studies and leads for identifying medically relevant protein targets. However, their use for these studies is often hampered by limited quantities and a lack of selective and mild monofunctionalization reactions. The development of selective methods that could simultaneously equip the natural product with a reactive group for subsequent conjugation to reporter tags and provide important structure-activity relationship (SAR) information, requiring only a knowledge of functional groups present in the natural product, could significantly decrease the time between bioactive natural product isolation and target identification. Herein, we report such a strategy that enables simultaneous arming and SAR studies of alcohol-containing natural products involving both chemo- and site-selective ("chemosite" selective) and site-nonselective O-H insertion reactions with rhodium carbenoids derived from alkynyl diazo acetates. This strategy was applied to a diverse set of natural products, and general guidelines for predicting chemosite selectivity were formulated. A subsequent Sharpless-Hüisgen [3 + 2] cycloaddition reaction with the appended alkyne allows for attachment of a variety of reporter tags. Using this strategy, we synthesized a novel FK506-biotin conjugate that enabled pull-down of the entire " immunosuppressive complex" including FKBP12, calcineurins A and B, and calmodulin. In addition, the potential for a chemoselective but site-nonselective process was shown with both gibberellic acid methyl ester and brefeldin A using only achiral rhodium catalysts.

Original languageEnglish (US)
Pages (from-to)12222-12231
Number of pages10
JournalJournal of the American Chemical Society
Volume129
Issue number40
DOIs
StatePublished - Oct 10 2007

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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