TY - JOUR
T1 - Simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres
AU - Mirro, Joseph
AU - Schwartz, Jill F.
AU - Civin, Curt I.
PY - 1981/11/30
Y1 - 1981/11/30
N2 - A new method for simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres ('immunospheres') is described. Wright's staining was performed on cells after reaction with immunospheres, and a direct correlation of cell surface antigen expression and cell morphology was made. Mild formalin fixation of cells inhibited phagocytosis of microspheres, which is a potential source of confusion in the analysis of cell surface binding. Rapid, accurate analysis of cell surface antigen expression in single cell suspensions of heterogeneous human hematopoietic and lymphoid cell populations was facilitated by this method.
AB - A new method for simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres ('immunospheres') is described. Wright's staining was performed on cells after reaction with immunospheres, and a direct correlation of cell surface antigen expression and cell morphology was made. Mild formalin fixation of cells inhibited phagocytosis of microspheres, which is a potential source of confusion in the analysis of cell surface binding. Rapid, accurate analysis of cell surface antigen expression in single cell suspensions of heterogeneous human hematopoietic and lymphoid cell populations was facilitated by this method.
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U2 - 10.1016/0022-1759(81)90255-6
DO - 10.1016/0022-1759(81)90255-6
M3 - Article
C2 - 7031139
AN - SCOPUS:0019814642
SN - 0022-1759
VL - 47
SP - 39
EP - 48
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -