Simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres

Joseph Mirro, Jill F. Schwartz, Curt I. Civin

Research output: Contribution to journalArticlepeer-review

Abstract

A new method for simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres ('immunospheres') is described. Wright's staining was performed on cells after reaction with immunospheres, and a direct correlation of cell surface antigen expression and cell morphology was made. Mild formalin fixation of cells inhibited phagocytosis of microspheres, which is a potential source of confusion in the analysis of cell surface binding. Rapid, accurate analysis of cell surface antigen expression in single cell suspensions of heterogeneous human hematopoietic and lymphoid cell populations was facilitated by this method.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalJournal of Immunological Methods
Volume47
Issue number1
DOIs
StatePublished - Nov 30 1981

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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