Simulating nirs and mrs measurements during cerebral hypoxia-ischaemia in piglets using a computational model

T. Hapuarachchi, T. Moroz, A. Bainbridge, S. Faulkner, D. Price, K. D. Broad, D. Thomas, E. Cady, X. Golay, Nicola Robertson, Ilias Tachtsidis

Research output: Contribution to journalArticlepeer-review

Abstract

We present a group analysis of the changes in cerebral haemodynamics, and the oxidation state of cytochrome-c-oxidase measured using broadband nearinfrared spectroscopy (NIRS) and intracellular pH measured by phosphorous (31 P) magnetic resonance spectroscopy (MRS) during and after cerebral hypoxiaischaemia (HI) in 15 piglets. We use a previously published computational model of cerebral metabolism in the piglet [1] to integrate these measurements and simulate HI. We successfully simulate changes in cellular metabolism including shifts in intracellular pH observed in the piglet brain during HI. In this process, we optimise physiological parameters in the model identifi ed through sensitivity analysis (such as the rate of glucose metabolism and intracellular lactate concentration), to fi t simulated and measured data. The model fi ts the data reasonably and suggests a 20% drop in glucose consumption, a ~65% increase in lactate concentration and ~35% drop in the cerebral metabolic rate of oxygen (CMRO 2) during HI.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalAdvances in experimental medicine and biology
Volume812
DOIs
StatePublished - 2014

Keywords

  • Intracellular pH
  • MRS
  • Mathematical modeling
  • Neonatal
  • Parameter optimization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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