Simplified method for isolation of intact avian and rat liver parencymal cells

David M. Capuzzi, Vicki Rothman, Simeon Margolis

Research output: Contribution to journalArticlepeer-review

Abstract

A new, simplified procedure is described for the isolation of intact liver cells from cockerel or rat liver. The technique involves a 15 min perfusion of the liver in situ through the portal vein with a solution of collagenase and hyaluronidase, and subsequent incubation of the minced liver in the enzyme solution. The yield of cells was excellent. Most cells excluded a vital stain and were undamaged when viewed with the electron microscope. The cells actively incorporated labeled precursors into lipids and proteins without specific cofactor requirements and responded to insulin in vitro. The biosynthetic capacity of the cells was retained for several days in culture.

Original languageEnglish (US)
Pages (from-to)421-429
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume45
Issue number2
DOIs
StatePublished - Oct 15 1971
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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