Similarity and difference in tumor-infiltrating lymphocytes in original tumor tissues and those of in vitro expanded populations in head and neck cancer

Lili Ren, Tatsuo Matsuda, Boya Deng, Kazuma Kiyotani, Taigo Kato, Jae Hyun Park, Tanguy Lim Seiwert, Everett E. Vokes, Nishant Agrawal, Yusuke Nakamura

Research output: Contribution to journalArticle

Abstract

Though adoptive tumor-infiltrating lymphocyte (TIL) therapy has been explored in clinical trials for many years, there is little information for the clonotype composition between TILs in original tumor tissues and TILs that were in vitro expanded and infused to cancer patients. To investigate the similarity/difference in TILs in original tumor tissues and those of in vitro expanded populations in squamous cell carcinoma of head and neck (SCCHN) as well as their correlation with somatic mutations in cancer cells, we performed whole exome analysis, expression profile analysis of immune-related genes, and T cell receptor (TCR) analysis of original TILs and in vitro expanded TILs in 8 surgically-resected HPV-negative fresh tumors with SCCHN. We found an unusually high number of non-synonymous somatic mutations (4290, 1779 and 901 mutations) in three SCCHN tumors, in which we identified mutations in mismatch repair genes, MSH2 or MSH4, or a DNA polymerase gene, POLE. Interestingly, dominant TCR clonotypes of expanded CD8+ TILs derived from these three tumors revealed high similarity to those in original tumors while for remaining tumors with the lower mutational load, we found that T cell clonotypes between TILs in original tumor tissues and those expanded in vitro were almost entirely different. Our findings might provide clinically useful information for identification of tumorantigen- specific T cell clones that may lead to further improvement of adoptive TIL therapy for SCCHN patients.

Original languageEnglish (US)
Pages (from-to)3805-3814
Number of pages10
JournalOncotarget
Volume9
Issue number3
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Tumor-Infiltrating Lymphocytes
Head and Neck Neoplasms
Population
Neoplasms
Mutation
In Vitro Techniques
Exome
T-Lymphocytes
T-Cell Receptor Genes
DNA Mismatch Repair
DNA-Directed DNA Polymerase
T-Cell Antigen Receptor
Genes
Clone Cells
Clinical Trials

Keywords

  • Mismatch repair
  • Non-synonymous mutation
  • Squamous cell carcinoma of head and neck cancer
  • T cell receptor
  • Tumor-infiltrating lymphocytes

ASJC Scopus subject areas

  • Oncology

Cite this

Similarity and difference in tumor-infiltrating lymphocytes in original tumor tissues and those of in vitro expanded populations in head and neck cancer. / Ren, Lili; Matsuda, Tatsuo; Deng, Boya; Kiyotani, Kazuma; Kato, Taigo; Park, Jae Hyun; Lim Seiwert, Tanguy; Vokes, Everett E.; Agrawal, Nishant; Nakamura, Yusuke.

In: Oncotarget, Vol. 9, No. 3, 01.01.2018, p. 3805-3814.

Research output: Contribution to journalArticle

Ren, Lili ; Matsuda, Tatsuo ; Deng, Boya ; Kiyotani, Kazuma ; Kato, Taigo ; Park, Jae Hyun ; Lim Seiwert, Tanguy ; Vokes, Everett E. ; Agrawal, Nishant ; Nakamura, Yusuke. / Similarity and difference in tumor-infiltrating lymphocytes in original tumor tissues and those of in vitro expanded populations in head and neck cancer. In: Oncotarget. 2018 ; Vol. 9, No. 3. pp. 3805-3814.
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