Simian hepatitis A virus (HAV) strain AGM-27: Comparison of genome structure and growth in cell culture with other HAV strains

S. A. Tsarev; S. U. Emerson; M. S. Balayan; J. Ticehurst; R. H. Purcell

doi:10.1099/0022-1317-72-7-1677

Simian hepatitis A virus (HAV) strain AGM-27: Comparison of genome structure and growth in cell culture with other HAV strains

S. A. Tsarev, S. U. Emerson, M. S. Balayan, J. Ticehurst, R. H. Purcell

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Abstract

Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

Original language	English (US)
Pages (from-to)	1677-1683
Number of pages	7
Journal	Journal of General Virology
Volume	72
Issue number	7
DOIs	https://doi.org/10.1099/0022-1317-72-7-1677
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "Simian hepatitis A virus (HAV) strain AGM-27: Comparison of genome structure and growth in cell culture with other HAV strains",

abstract = "Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.",

author = "Tsarev, {S. A.} and Emerson, {S. U.} and Balayan, {M. S.} and J. Ticehurst and Purcell, {R. H.}",

year = "1991",

doi = "10.1099/0022-1317-72-7-1677",

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T1 - Simian hepatitis A virus (HAV) strain AGM-27

T2 - Comparison of genome structure and growth in cell culture with other HAV strains

AU - Tsarev, S. A.

AU - Emerson, S. U.

AU - Balayan, M. S.

AU - Ticehurst, J.

AU - Purcell, R. H.

PY - 1991

Y1 - 1991

N2 - Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

AB - Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

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Simian hepatitis A virus (HAV) strain AGM-27: Comparison of genome structure and growth in cell culture with other HAV strains

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