Simian hepatitis A virus (HAV) strain AGM-27: Comparison of genome structure and growth in cell culture with other HAV strains

S. A. Tsarev, S. U. Emerson, M. S. Balayan, J. Ticehurst, R. H. Purcell

Research output: Contribution to journalArticlepeer-review

Abstract

Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

Original languageEnglish (US)
Pages (from-to)1677-1683
Number of pages7
JournalJournal of General Virology
Volume72
Issue number7
DOIs
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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