Silver-Zinc Redox-Coupled electroceutical wound dressing disrupts bacterial biofilm

Banerjee Jaideep, Das Ghatak Piya, Roy Sashwati, Khanna Savita, Hemann Craig, Deng Binbin, Das Amitava, L. Zweier Jay, Wozniak Daniel, K. Sen Chandan

Research output: Contribution to journalArticle

Abstract

Pseudomonas aeruginosa biofilm is commonly associated with chronic wound infection. A FDA approved wireless electroceutical dressing (WED), which in the presence of conductive wound exudate gets activated to generate electric field (0.3 -0.9V), was investigated for its anti-biofilm properties. Growth of pathogenic P. aeruginosa strain PAO1 in LB media was markedly arrested in the presence of the WED. Scanning electron microscopy demonstrated that WED markedly disrupted biofilm integrity in a setting where silver dressing was ineffective. Biofilm thickness and number of live bacterial cells were decreased in the presence of WED. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. This work provides first electron paramagnetic resonance spectroscopy evidence demonstrating that WED serves as a spontaneous source of reactive oxygen species. Redox-sensitive multidrug efflux systems mexAB and mexEF were repressed by WED. Taken together, these observations provide first evidence supporting the anti-biofilm properties of WED.

Original languageEnglish (US)
Article numbere0119531
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 24 2015
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Silver-Zinc Redox-Coupled electroceutical wound dressing disrupts bacterial biofilm'. Together they form a unique fingerprint.

  • Cite this

    Jaideep, B., Piya, D. G., Sashwati, R., Savita, K., Craig, H., Binbin, D., Amitava, D., Jay, L. Z., Daniel, W., & Chandan, K. S. (2015). Silver-Zinc Redox-Coupled electroceutical wound dressing disrupts bacterial biofilm. PLoS One, 10(3), [e0119531]. https://doi.org/10.1371/journal.pone.0119531