Silver staining of histones in Triton-acid-urea gels

David E. Mold; Jon Weingart; Joel Assaraf; Dennis B. Lubahn; Drew N. Kelner; Barbara Ramsay Shaw; Kenneth S. McCarty

doi:10.1016/0003-2697(83)90727-3

Silver staining of histones in Triton-acid-urea gels

David E. Mold, Jon Weingart, Joel Assaraf, Dennis B. Lubahn, Drew N. Kelner, Barbara Ramsay Shaw, Kenneth S. McCarty

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.

Original language	English (US)
Pages (from-to)	44-47
Number of pages	4
Journal	Analytical Biochemistry
Volume	135
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(83)90727-3
State	Published - Nov 1983
Externally published	Yes

Keywords

Triton-acid-urea gels
amido black
electrophoresis
histone
protein fixation
silver stain

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(83)90727-3

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title = "Silver staining of histones in Triton-acid-urea gels",

abstract = "A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.",

keywords = "Triton-acid-urea gels, amido black, electrophoresis, histone, protein fixation, silver stain",

author = "Mold, {David E.} and Jon Weingart and Joel Assaraf and Lubahn, {Dennis B.} and Kelner, {Drew N.} and Shaw, {Barbara Ramsay} and McCarty, {Kenneth S.}",

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language = "English (US)",

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T1 - Silver staining of histones in Triton-acid-urea gels

AU - Mold, David E.

AU - Weingart, Jon

AU - Assaraf, Joel

AU - Lubahn, Dennis B.

AU - Kelner, Drew N.

AU - Shaw, Barbara Ramsay

AU - McCarty, Kenneth S.

PY - 1983/11

Y1 - 1983/11

N2 - A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.

AB - A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.

KW - Triton-acid-urea gels

KW - amido black

KW - electrophoresis

KW - histone

KW - protein fixation

KW - silver stain

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Silver staining of histones in Triton-acid-urea gels

Abstract

Keywords

ASJC Scopus subject areas

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