Silver staining of histones in Triton-acid-urea gels

David E. Mold, Jon Weingart, Joel Assaraf, Dennis B. Lubahn, Drew N. Kelner, Barbara Ramsay Shaw, Kenneth S. McCarty

Research output: Contribution to journalArticlepeer-review

Abstract

A reliable method for silver staining histones in Triton-acid-urea gels was developed. Optimum staining is achieved by treating the gels either with amido black or a colorless, water-soluble analog of amido black, 2,7-naphthalenedisulfonic acid, prior to staining with ammoniacal silver. Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level. The use of 2,7-naphthalenedisulfonic acid dramatically shortens the procedure permitting histone patterns to be visualized within 5 h.

Original languageEnglish (US)
Pages (from-to)44-47
Number of pages4
JournalAnalytical Biochemistry
Volume135
Issue number1
DOIs
StatePublished - Nov 1983
Externally publishedYes

Keywords

  • Triton-acid-urea gels
  • amido black
  • electrophoresis
  • histone
  • protein fixation
  • silver stain

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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