TY - JOUR
T1 - Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury. Immunohistochemical study in rat model
AU - Armstrong, Dinani Matoso Fialho de Oliveira
AU - Armstrong, Anderson da Costa
AU - Figueiredo, Regina Célia Bressan Queiroz
AU - Florentino, Joao Eduardo
AU - Saad, Paulo Fernandes
AU - Fox-Talbot, Karen
AU - Halushka, Marc Kenneth
AU - Berkowitz, Dan E.
AU - Taha, Murched Omar
AU - Fagundes, Djalma José
PY - 2013/4
Y1 - 2013/4
N2 - PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
AB - PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
KW - Caspase 3
KW - Muscle
KW - Phosphodiesterase 5 inhibitors
KW - Rats
KW - Reperfusion in0jury
KW - Skeletal
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U2 - 10.1590/S0102-86502013000400008
DO - 10.1590/S0102-86502013000400008
M3 - Article
C2 - 23568236
AN - SCOPUS:84876940708
SN - 0102-8650
VL - 28
SP - 282
EP - 287
JO - Acta cirurgica brasileira
JF - Acta cirurgica brasileira
IS - 4
ER -