Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury. Immunohistochemical study in rat model

Dinani Matoso Fialho de Oliveira Armstrong, Anderson da Costa Armstrong, Regina Célia Bressan Queiroz Figueiredo, Joao Eduardo Florentino, Paulo Fernandes Saad, Karen Fox-Talbot, Marc Kenneth Halushka, Dan E. Berkowitz, Murched Omar Taha, Djalma José Fagundes

Research output: Contribution to journalArticle

Abstract

PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalActa cirurgica brasileira
Volume28
Issue number4
DOIs
StatePublished - Apr 2013

Keywords

  • Caspase 3
  • Muscle
  • Phosphodiesterase 5 inhibitors
  • Rats
  • Reperfusion in0jury
  • Skeletal

ASJC Scopus subject areas

  • Surgery

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  • Cite this

    Armstrong, D. M. F. D. O., Armstrong, A. D. C., Figueiredo, R. C. B. Q., Florentino, J. E., Saad, P. F., Fox-Talbot, K., Halushka, M. K., Berkowitz, D. E., Taha, M. O., & Fagundes, D. J. (2013). Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury. Immunohistochemical study in rat model. Acta cirurgica brasileira, 28(4), 282-287. https://doi.org/10.1590/S0102-86502013000400008