TY - JOUR
T1 - SILAC for global phosphoproteomic analysis.
AU - Pimienta, Genaro
AU - Chaerkady, Raghothama
AU - Pandey, Akhilesh
PY - 2009
Y1 - 2009
N2 - Establishing the phosphorylation pattern of proteins in a comprehensive fashion is an important goal of a majority of cell signaling projects. Phosphoproteomic strategies should be designed in such a manner as to identify sites of phosphorylation as well as to provide quantitative information about the extent of phosphorylation at the sites. In this chapter, we describe an experimental strategy that outlines such an approach using stable isotope labeling with amino acids in cell culture (SILAC) coupled to LC-MS/MS. We highlight the importance of quantitative strategies in signal transduction as a platform for a systematic and global elucidation of biological processes.
AB - Establishing the phosphorylation pattern of proteins in a comprehensive fashion is an important goal of a majority of cell signaling projects. Phosphoproteomic strategies should be designed in such a manner as to identify sites of phosphorylation as well as to provide quantitative information about the extent of phosphorylation at the sites. In this chapter, we describe an experimental strategy that outlines such an approach using stable isotope labeling with amino acids in cell culture (SILAC) coupled to LC-MS/MS. We highlight the importance of quantitative strategies in signal transduction as a platform for a systematic and global elucidation of biological processes.
UR - http://www.scopus.com/inward/record.url?scp=65349090678&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349090678&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-834-8_9
DO - 10.1007/978-1-60327-834-8_9
M3 - Review article
C2 - 19241009
AN - SCOPUS:65349090678
SN - 1064-3745
VL - 527
SP - 107-116, x
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -