Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents

M. M. Nicolle, J. Gonzalez, K. Sugaya, K. A. Baskerville, D. Bryan, K. Lund, Michela Gallagher, M. McKinney

Research output: Contribution to journalArticle

Abstract

Neurons and glia within the hippocampus of aged, spatial learning-impaired Long-Evans rats exhibit uniquely altered gene expression profiles, and we have postulated oxidative stress as the basis for this. To test this hypothesis we quantitated the extent of protein and nucleic acid oxidative damage, evaluated the status of mitochondrial DNA integrity, and examined several signaling entities and molecular indicators frequently associated with oxidative stress and gliosis. Immunoblotting demonstrated elevated heme oxygenase-1 in the aged-impaired hippocampus and immunocytochemistry suggested that heme oxygenase-1 is largely cytosolic and at least partly neuronal in nature. In the aged-impaired group, immunoreactivity to 8-hydroxy-2′-deoxyguanosine, an oxidative nucleic acid adduct, was found to be elevated in the dentate gyrus and in area CA1 of the hippocampal formation. Isolated mitochondrial DNA was found to be significantly damaged in the aged-impaired group. In the aged learning-impaired rats only, proteins in a 65-kDa band were found to contain excessive levels of carbonyl residues. Glial activation was examined by in situ hybridization histochemistry to tumor necrosis factor α and by immunocytochemistry with OX-6, which detects activated microglia. White matter in aged brains exhibited a modest up-regulation of tumor necrosis factor α mRNA and OX-6 immunoreactivity, but the hippocampal formation expressed tumor necrosis factor α mRNA equivalent to young animals and few OX-6-positive microglia. The mRNA for manganese-dependent superoxide dismutase, which is elevated in the aged hippocampus, was found preferentially expressed in neurons. We conclude that aged hippocampal neurons appear to be under oxidative stress and this is more severe in the learning-impaired subjects, suggesting a possible basis for age-induced cognitive decline.

Original languageEnglish (US)
Pages (from-to)415-431
Number of pages17
JournalNeuroscience
Volume107
Issue number3
DOIs
StatePublished - Nov 23 2001

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Rodentia
Hippocampus
Oxidative Stress
Heme Oxygenase-1
Tumor Necrosis Factor-alpha
Microglia
Mitochondrial DNA
Neurons
Neuroglia
Messenger RNA
Nucleic Acids
Immunohistochemistry
Learning
Long Evans Rats
Gliosis
Dentate Gyrus
Transcriptome
Immunoblotting
Superoxide Dismutase
In Situ Hybridization

Keywords

  • Aging
  • Cognition
  • Cytokines
  • Hippocampus
  • Mitochondria
  • Oxidative damage

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nicolle, M. M., Gonzalez, J., Sugaya, K., Baskerville, K. A., Bryan, D., Lund, K., ... McKinney, M. (2001). Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents. Neuroscience, 107(3), 415-431. https://doi.org/10.1016/S0306-4522(01)00374-8

Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents. / Nicolle, M. M.; Gonzalez, J.; Sugaya, K.; Baskerville, K. A.; Bryan, D.; Lund, K.; Gallagher, Michela; McKinney, M.

In: Neuroscience, Vol. 107, No. 3, 23.11.2001, p. 415-431.

Research output: Contribution to journalArticle

Nicolle, MM, Gonzalez, J, Sugaya, K, Baskerville, KA, Bryan, D, Lund, K, Gallagher, M & McKinney, M 2001, 'Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents', Neuroscience, vol. 107, no. 3, pp. 415-431. https://doi.org/10.1016/S0306-4522(01)00374-8
Nicolle MM, Gonzalez J, Sugaya K, Baskerville KA, Bryan D, Lund K et al. Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents. Neuroscience. 2001 Nov 23;107(3):415-431. https://doi.org/10.1016/S0306-4522(01)00374-8
Nicolle, M. M. ; Gonzalez, J. ; Sugaya, K. ; Baskerville, K. A. ; Bryan, D. ; Lund, K. ; Gallagher, Michela ; McKinney, M. / Signatures of hippocampal oxidative stress in aged spatial learning-impaired rodents. In: Neuroscience. 2001 ; Vol. 107, No. 3. pp. 415-431.
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