TY - JOUR
T1 - Side chain dynamics in unfolded protein states
T2 - An NMR based 2H spin relaxation study of Δ131Δ
AU - Choy, Wing Yiu
AU - Shortle, David
AU - Kay, Lewis E.
PY - 2003/2/15
Y1 - 2003/2/15
N2 - NMR relaxation data on disordered proteins can provide insight into both structural and dynamic properties of these molecules. Because of chemical shift degeneracy in correlation spectra, detailed sitespecific analyses of side chain dynamics have not been possible. Here, we present new experiments for the measurement of side chain dynamics in methyl-containing residues in unfolded protein states. The pulse schemes are similar to recently proposed methods for measuring deuterium spin relaxation rates in 13CH2D methyl groups in folded proteins.1 However, because resolution in 1H-13C correlation maps of unfolded proteins is limiting, relaxation data are recorded as a series of 1H-15N spectra. The methodology is illustrated with an application to the study of side chain dynamics in Δ131Δ, a large disordered fragment of staphylococcal nuclease containing residues 1-3 and 13-140 of the wide-type protein. A good correlation between the order parameters of the symmetry axes of the methyl groups and the backbone 1H-15N bond vectors of the same residue is observed. Simulations establish that such a correlation is only possible if the unfolded state is comprised of an ensemble of structures which are not equiprobable. A motional model, which combines wobbling-in-a-cone and Gaussian axial fluctuations, is proposed to estimate χ1 torsion angle fluctuations, σχ1, of Val and Thr residues on the basis of the backbone and side chain order parameters. Values of σχ1 are approximately 10° larger than what has previously been observed in folded proteins. Of interest, the value of σχ1 for Val 104 is considerably smaller than for other Val or Thr residues, suggesting that it may be part of a hydrophobic cluster. Notably large 15N transverse relaxation rates are observed in this region. To our knowledge, this is the first time that side chain dynamics in an unfolded state have been studied in detail by NMR.
AB - NMR relaxation data on disordered proteins can provide insight into both structural and dynamic properties of these molecules. Because of chemical shift degeneracy in correlation spectra, detailed sitespecific analyses of side chain dynamics have not been possible. Here, we present new experiments for the measurement of side chain dynamics in methyl-containing residues in unfolded protein states. The pulse schemes are similar to recently proposed methods for measuring deuterium spin relaxation rates in 13CH2D methyl groups in folded proteins.1 However, because resolution in 1H-13C correlation maps of unfolded proteins is limiting, relaxation data are recorded as a series of 1H-15N spectra. The methodology is illustrated with an application to the study of side chain dynamics in Δ131Δ, a large disordered fragment of staphylococcal nuclease containing residues 1-3 and 13-140 of the wide-type protein. A good correlation between the order parameters of the symmetry axes of the methyl groups and the backbone 1H-15N bond vectors of the same residue is observed. Simulations establish that such a correlation is only possible if the unfolded state is comprised of an ensemble of structures which are not equiprobable. A motional model, which combines wobbling-in-a-cone and Gaussian axial fluctuations, is proposed to estimate χ1 torsion angle fluctuations, σχ1, of Val and Thr residues on the basis of the backbone and side chain order parameters. Values of σχ1 are approximately 10° larger than what has previously been observed in folded proteins. Of interest, the value of σχ1 for Val 104 is considerably smaller than for other Val or Thr residues, suggesting that it may be part of a hydrophobic cluster. Notably large 15N transverse relaxation rates are observed in this region. To our knowledge, this is the first time that side chain dynamics in an unfolded state have been studied in detail by NMR.
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U2 - 10.1021/ja021179b
DO - 10.1021/ja021179b
M3 - Article
C2 - 12580600
AN - SCOPUS:0037442335
VL - 125
SP - 1748
EP - 1758
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 7
ER -