Sickled erythrocytes, hyphema, and secondary glaucoma. IV. The rate and percentage of sickling of erythrocytes in rabbit aqueous humor, in vitro and in vivo

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Abstract

The number of erythrocytes adopting the sickled configuration in rabbit aqueous was determined with a masked counting technique in vitro and in vivo. The percentage of cells that became sickled in aqueous humor was greater than that observed in a control salt solution and in the donor blood itself. Aqueous humor, therefore, can be considered a particularly deleterious medium for erythrocytes having a propensity for sickling. Blood samples from six donors with systemically severe hemoglobinopathies (SC, Sthal, and SS) had relatively high levels of hemoglobin S and, in general, showed faster and more frequent sickling than did blood samples from donors with low levels of hemoglobin S (one SS patient, whose chronic blood transfusion regimen had replaced most of his hemoglobin S with normal hemoglobin, and two AS patients). Even erythrocytes from sickle cell trait cases, however, were capable of sickling when immersed in aqueous humor.

Original languageEnglish (US)
Pages (from-to)62-69
Number of pages8
JournalOphthalmic surgery
Volume10
Issue number4
StatePublished - Dec 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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