Sickle cell imaging flow cytometry assay (SIFCA)

Kleber Y. Fertrin, Leigh Samsel, Eduard J. Van Beers, Laurel Mendelsohn, Gregory J. Kato, J. Philip McCoy

Research output: Contribution to journalArticlepeer-review


Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.

Original languageEnglish (US)
Pages (from-to)279-292
Number of pages14
JournalMethods in Molecular Biology
StatePublished - 2016


  • Imaging flow cytometry
  • Sickle cell disease
  • Sickling assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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