Sickle and thalassemic erythroid progenitor cells are different from normal

Blanche P. Alter, Liya He, Robert Acosta, Mary Ellen Knobloch, Joann C. Thomson, Patricia Giardina, Rona S. Weinberg

Research output: Contribution to journalArticle

Abstract

Blood erythroid progenitors (BFU-E) from patients with sickle and thalassemic syndromes were compared with those from normal individuals. The day of maximal colony formation in methyl cellulose was slightly later in the cultures from the patients with hemoglobinopathies than in the normal cultures. The number of colonies/100,000 mononuclear cells was similar in all cultures on day 13, but was higher in the hemoglobinopathy cultures on the day of maximal growth. The number of BFU-E/mL of blood was significantly higher than normal at all times in both sickle cell anemia and thalassemia. The proportional synthesis of γ globin was twice normal in all sickle cultures, and 4 times normal in those from β+-thalassemia. Hemin and interleukin-3 increased the numbers of erythroid colonies in all cultures, but did not consistently alter the globin synthesis patterns. Each progenitor population has a unique pattern in terms of time course, number of BFU-E, and level of γ globin synthesis. These features indicate distinct types of BFU-E, or differences in accessory cells, or both, which distinguish blood-borne erythropoiesis in normals and those with hemoglobinopathies.

Original languageEnglish (US)
Pages (from-to)447-467
Number of pages21
JournalHemoglobin
Volume16
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Erythroid Precursor Cells
Globins
Hemoglobinopathies
Blood
Thalassemia
Hemin
Methylcellulose
Interleukin-3
Accessories
Erythropoiesis
Sickle Cell Anemia
Growth
Population

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)
  • Clinical Biochemistry
  • Biochemistry, medical
  • Biochemistry

Cite this

Alter, B. P., He, L., Acosta, R., Knobloch, M. E., Thomson, J. C., Giardina, P., & Weinberg, R. S. (1992). Sickle and thalassemic erythroid progenitor cells are different from normal. Hemoglobin, 16(6), 447-467. https://doi.org/10.3109/03630269208993114

Sickle and thalassemic erythroid progenitor cells are different from normal. / Alter, Blanche P.; He, Liya; Acosta, Robert; Knobloch, Mary Ellen; Thomson, Joann C.; Giardina, Patricia; Weinberg, Rona S.

In: Hemoglobin, Vol. 16, No. 6, 1992, p. 447-467.

Research output: Contribution to journalArticle

Alter, BP, He, L, Acosta, R, Knobloch, ME, Thomson, JC, Giardina, P & Weinberg, RS 1992, 'Sickle and thalassemic erythroid progenitor cells are different from normal', Hemoglobin, vol. 16, no. 6, pp. 447-467. https://doi.org/10.3109/03630269208993114
Alter BP, He L, Acosta R, Knobloch ME, Thomson JC, Giardina P et al. Sickle and thalassemic erythroid progenitor cells are different from normal. Hemoglobin. 1992;16(6):447-467. https://doi.org/10.3109/03630269208993114
Alter, Blanche P. ; He, Liya ; Acosta, Robert ; Knobloch, Mary Ellen ; Thomson, Joann C. ; Giardina, Patricia ; Weinberg, Rona S. / Sickle and thalassemic erythroid progenitor cells are different from normal. In: Hemoglobin. 1992 ; Vol. 16, No. 6. pp. 447-467.
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