Short- vs. long-circulating magnetoliposomes as bone marrow-seeking MR contrast agents

Jeff W Bulte, Marcel De Cuyper, Daryl Despres, Joseph A. Frank

Research output: Contribution to journalArticle

Abstract

We evaluated the relaxation enhancement and biodistribution of short- vs. long-circulating magnetoliposomes as a new contrast agent for magnetic resonance (MR) imaging of bone marrow. Magnetoliposomes with (ML-PEG) and without (ML) incorporation of polyethylene glycol (PEG, Mw 2000) were prepared, measuring 40 nm in diameter with 1-6 iron oxide crystals/vesicle. PEGylation selectively enhanced the T2 relaxivity of magnetoliposomes by 10% to 15%, with R1 and R2 values of 3 and 240 s-1/mM at 1.5 T and 37°C. ML (n = 6) and ML-PEG (n = 6) preparations were administered IV into young (6-8 weeks old) and adult (> 1 year old) Sprague-Dawley rats at 100 μmol Fe/kg. PEGylation increased blood half-life (P <0.05 for t > 30 minutes), following a biexponential clearance with a long half-life of 53.2 ± 13.2 minutes. The clearance of ML was monoexponential, with a half-life 7.4 ± 0.4 minutes. MR imaging revealed a pronounced uptake in bone marrow, including the iliac bone, femur, tibia, and upper and lower vertebrae. The bone marrow uptake of ML-PEG was comparable to that of ML, with both reaching a plateau within 30 minutes following injection. Fast spin-echo T2-weighted imaging was found to provide optimal contrast enhancement and allowed a clear depiction of red to yellow marrow conversion due to normal aging. While the use of magnetoliposomes can provide the added benefit of therapeutic drug or gene delivery, further investigation is warranted to assess their usefulness in differentiating normal vs. abnormal marrow conditions.

Original languageEnglish (US)
Pages (from-to)329-335
Number of pages7
JournalJournal of Magnetic Resonance Imaging
Volume9
Issue number2
DOIs
StatePublished - Feb 1999
Externally publishedYes

Fingerprint

Contrast Media
Magnetic Resonance Spectroscopy
Bone Marrow
Half-Life
Magnetic Resonance Imaging
Tibia
Femur
Sprague Dawley Rats
Spine
Bone and Bones
Injections
Pharmaceutical Preparations
Genes
Therapeutics

Keywords

  • Bone marrow
  • Liposome
  • MR imaging
  • Superparamagnetic iron oxide

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Short- vs. long-circulating magnetoliposomes as bone marrow-seeking MR contrast agents. / Bulte, Jeff W; De Cuyper, Marcel; Despres, Daryl; Frank, Joseph A.

In: Journal of Magnetic Resonance Imaging, Vol. 9, No. 2, 02.1999, p. 329-335.

Research output: Contribution to journalArticle

Bulte, Jeff W ; De Cuyper, Marcel ; Despres, Daryl ; Frank, Joseph A. / Short- vs. long-circulating magnetoliposomes as bone marrow-seeking MR contrast agents. In: Journal of Magnetic Resonance Imaging. 1999 ; Vol. 9, No. 2. pp. 329-335.
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