TY - JOUR
T1 - Short-Term Stability of Hematologic Parameters in Frozen Whole Blood
AU - Tang, Olive
AU - Selvin, Elizabeth
AU - Arends, Valerie
AU - Saenger, Amy
N1 - Publisher Copyright:
© 2019 American Association for Clinical Chemistry.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Background: Complete blood counts (CBCs) are commonly obtained in large multicenter studies. We assessed the stability of 10 parameters after short-term (up to 30 days) frozen storage. Methods: We compared CBC measurements from fresh samples (n = 53) with samples stored for up to 30 days at -70 °C. We calculated the CVs and intraclass correlation coefficients. Results: Mean values of most parameters, with the exception of hemoglobin and platelet count, were significantly different by 15 days of storage. White blood cell count (CV, 38.3%; 95% CI, 31.3%- 46.2%) and red cell distribution width (CV, 37.7%; 95% CI, 34.1%- 41.3%) were the most variable. After 30 days, only hemoglobin remained stable and reliable (CV, 0.8%; 95% CI, 0.4%-1.3%). Conclusions: Hemoglobin remained stable in frozen blood samples stored for up to 30 days at -70 °C and may be reliably used in research studies using short-term frozen specimens. Other CBC parameters measured in stored blood are not sufficiently reliable for research or patient care.
AB - Background: Complete blood counts (CBCs) are commonly obtained in large multicenter studies. We assessed the stability of 10 parameters after short-term (up to 30 days) frozen storage. Methods: We compared CBC measurements from fresh samples (n = 53) with samples stored for up to 30 days at -70 °C. We calculated the CVs and intraclass correlation coefficients. Results: Mean values of most parameters, with the exception of hemoglobin and platelet count, were significantly different by 15 days of storage. White blood cell count (CV, 38.3%; 95% CI, 31.3%- 46.2%) and red cell distribution width (CV, 37.7%; 95% CI, 34.1%- 41.3%) were the most variable. After 30 days, only hemoglobin remained stable and reliable (CV, 0.8%; 95% CI, 0.4%-1.3%). Conclusions: Hemoglobin remained stable in frozen blood samples stored for up to 30 days at -70 °C and may be reliably used in research studies using short-term frozen specimens. Other CBC parameters measured in stored blood are not sufficiently reliable for research or patient care.
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U2 - 10.1373/jalm.2018.028357
DO - 10.1373/jalm.2018.028357
M3 - Article
C2 - 31659078
AN - SCOPUS:85091192652
SN - 2576-9456
VL - 4
SP - 410
EP - 414
JO - Journal of Applied Laboratory Medicine
JF - Journal of Applied Laboratory Medicine
IS - 3
ER -