Short telomeres induce a DNA damage response in Saccharomyces cerevisiae

Arne S. IJpma, Carol W Greider

Research output: Contribution to journalArticle

Abstract

Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Δ cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rad53p phosphorylation. These responses were dependent on RAD24 and MEC1, suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.

Original languageEnglish (US)
Pages (from-to)987-1001
Number of pages15
JournalMolecular Biology of the Cell
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Telomere
DNA Damage
Saccharomyces cerevisiae
Telomerase
Growth
Homologous Recombination
Chromosomes
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Short telomeres induce a DNA damage response in Saccharomyces cerevisiae. / IJpma, Arne S.; Greider, Carol W.

In: Molecular Biology of the Cell, Vol. 14, No. 3, 01.03.2003, p. 987-1001.

Research output: Contribution to journalArticle

@article{98495f6b032549c5be6c5a72b77fe8bb,
title = "Short telomeres induce a DNA damage response in Saccharomyces cerevisiae",
abstract = "Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Δ cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rad53p phosphorylation. These responses were dependent on RAD24 and MEC1, suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.",
author = "IJpma, {Arne S.} and Greider, {Carol W}",
year = "2003",
month = "3",
day = "1",
doi = "10.1091/mbc.02-04-0057",
language = "English (US)",
volume = "14",
pages = "987--1001",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "3",

}

TY - JOUR

T1 - Short telomeres induce a DNA damage response in Saccharomyces cerevisiae

AU - IJpma, Arne S.

AU - Greider, Carol W

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Δ cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rad53p phosphorylation. These responses were dependent on RAD24 and MEC1, suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.

AB - Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Δ cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rad53p phosphorylation. These responses were dependent on RAD24 and MEC1, suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.

UR - http://www.scopus.com/inward/record.url?scp=0037341611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037341611&partnerID=8YFLogxK

U2 - 10.1091/mbc.02-04-0057

DO - 10.1091/mbc.02-04-0057

M3 - Article

VL - 14

SP - 987

EP - 1001

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 3

ER -