Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens

Sean Fitzwater, Maritza Calderon, Carlos LaFuente, Gerson Galdos-Cardenas, Lisbeth Ferrufino, Manuela Verastegui, Robert H. Gilman, Caryn Bern

Research output: Contribution to journalArticlepeer-review

Abstract

Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

Original languageEnglish (US)
Pages (from-to)768-770
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number5
DOIs
StatePublished - Nov 2008

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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