TY - JOUR
T1 - Short report
T2 - Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens
AU - Fitzwater, Sean
AU - Calderon, Maritza
AU - LaFuente, Carlos
AU - Galdos-Cardenas, Gerson
AU - Ferrufino, Lisbeth
AU - Verastegui, Manuela
AU - Gilman, Robert H.
AU - Bern, Caryn
PY - 2008/11
Y1 - 2008/11
N2 - Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.
AB - Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.
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UR - http://www.scopus.com/inward/citedby.url?scp=55849112284&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.2008.79.768
DO - 10.4269/ajtmh.2008.79.768
M3 - Article
C2 - 18981520
AN - SCOPUS:55849112284
SN - 0002-9637
VL - 79
SP - 768
EP - 770
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -