Short forms of Ste20-related proline/alanine-rich kinase (SPAK) in the kidney are created by aspartyl aminopeptidase (Dnpep)-mediated proteolytic cleavage

Nicolas Markadieu, Kerri Rios, Benjamin W. Spiller, W. Hayes McDonald, Paul A. Welling, Eric Delpire

Research output: Contribution to journalArticle

Abstract

Background: C-terminal SPAK fragments are found in kidney medulla.

Results:Weidentified Dnpep as the protease responsible for SPAK cleavage, identified the sites of cleavage, and showed unusual preference for α helices.

Conclusion: C-terminal SPAK fragments originate from Dnpep-mediated proteolytic cleavage.

Significance: SPAK and its cleavage are significant for the regulation of renal Na+ reabsorption and control of blood pressure.

Original languageEnglish (US)
Pages (from-to)29273-29284
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number42
DOIs
StatePublished - Oct 17 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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